This paper describes the effects of different varieties and production methods on yield and vitamin C content in lettuce leaves produced in the winter period. A three- year study was conducted by randomized block system in a greenhouse without additional heating. The trial included three varieties of lettuce (Archimeds RZ, RZ Santoro, Kibo RZ). Each row with these varieties was exposed to the following variants of covering: control-planting on bare soil, mulching before sowing with PE-black foil, agro textile- covering plants after planting with agro textile (17 grams), a combination of mulching + agro textile. The results show that the different methods of production affected the yield of lettuce. The highest yield was recorded in agro textile variant (14 kg/10m2), and lowest in controls (9.31 kg/10m2). Throughout of all the three years of the trial, it was continuously evidenced that the variety Santoro had the biggest heads and the highest yield (15.33 kg/10m2), which leads to conclusion that the yield of lettuce is a varietal characteristics. Measuring the content of vitamin C has shown that, depending on the method of production, i.e. applied different variants of coverage differ. In average, the combination of mulching + agro textile (26.77 mg/100 g) had the highest content while the control variant had significantly lower vitamin C content (21.10 mg/100 g).