2014, vol. 68, br. 2, str. 193-198
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Antioksidativni kapacitet i sadržaj fenola, askorbinske kiseline, β-karotena i likopena u salati
Antioxidant capacity and contents of phenols, ascorbic acid, β-carotene and lycopene in lettuce
Projekat: Novi koncept oplemenjivanja sorti i hibrida povrća namenjenih održivim sistemima gajenja uz primenu biotehnoloških metoda (MPNTR - 31059)
Sažetak
Određena je antioksidativna aktivnost tri sorte salate (Lactuca sativa L.) Emerald, Vera i Neva gajene u dve vrste zaštićenog prostora stakleniku i plasteniku pri kontrolisanim uslovima. Sadržaji antioksidativnih komponenti: ukupni fenoli, flavonoidi, L-askorbinska kiselina, β-karoten i likopen, određeni su u etanolskim ekstraktima salata spektrofotometrijskim metodama. Dobijeno je da najveći sadržaj ukupnih fenola (78,98 ± 0,67 mg GAE/g suvog ekstrakta) ima etanolski ekstrakt salate sorte Neva gajene u plasteniku, dok salata Emerald gajena u stakleniku ima najveći sadržaj flavonoida (35,45±0,95 mg RU/g suvog ekstrakta). Zapaženo je da salate gajene u stakleniku imaju nešto veći sadržaj L-askorbinske kiseline od salate istih sorti iz plastenika. Sadržaj likopena u ispitivanim salatama je zanemarljiv, a β-karotena je nizak. S druge strane visok sadržaj fenolnih komponenti uzrokuju dobre antioksidativne osobine nađene u svim sortama ispitivanih salata.
Abstract
The antioxidant activity of three lettuce varieties (Lactuca sativa L.) Emerald, Vera and Neva, cultivated in two kinds of protected spaces, a glasshouse and a plastic greenhouse, under controlled conditions, was determined. The content of antioxidant compounds: total phenols, flavonoids, L-ascorbic acid, β-carotene and lycopene, were determined in ethanolic extracts of the lettuce with spectrophotometric methods. The largest content of total phenols (78.98±0.67 mg GAE/g of dry extract) was found in ethanolic extract of the lettuce variety Neva cultivated in a plastic greenhouse, whereas the largest content of flavonoids (35.45±0.95 mg RU/g of dry extract) was displayed in the lettuce Emerald cultivated in a glasshouse. It was observed that the lettuce cultivated in the glasshouse contained a somewhat higher content of L-ascorbic acid than the lettuce same variety from plastic greenhouse. The content of lycopene in the examined lettuce is negligible, and the content of β-carotene is low. On the other hand, the high content of phenolic components causes favourable antioxidant properties found in all varieties of examined lettuce.
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